Journal: PLoS ONE

Article Title: Demineralized bone matrix used for direct pulp capping in rats

doi: 10.1371/journal.pone.0172693

Figure Lengend Snippet: (A): Representative immunohistochemical images of the blank control group, the Ca(OH) 2 group and the DBM group. a: The blank control group. Pulp morphology was normal. b- f: Ca(OH) 2 group (1, 3, 7, 14, 28 days). g- k: DBM group (1, 3, 7, 14, 28 days). (B): Mean IOD value of Runx2. *means significant difference.

Article Snippet: The sections were deparaffinized with xylene, hydrated in a series of descending grades of ethanol, and then rinsed briefly with PBS for the primary antibodies; the sections were incubated overnight at 4°C with polyclonal anti- Runx2, COL I, OCN and DSP (Wuhan Boster Biological Technology, Wuhan, China).

Techniques: Immunohistochemical staining, Control

Journal: British Journal of Cancer

Article Title: Breast osteoblast-like cells: a new biomarker for the management of breast cancer

doi: 10.1038/s41416-018-0255-y

Figure Lengend Snippet: Breast osteoblast-like cells (BOLCs) and the expression of breast cancer biomarkers. a Graph shows the Nottingham histological score of breast-infiltrating carcinomas. b Graph displays the number of BOLCs in G1, G2 and G3 groups (Nottingham score). c Image shows BOLCs in infiltrating breast cancer; RANKL expression Texas-Red, RUNX2 expression FITC (scale bar represents 20 µm). d High magnification of BOLCs; RANKL expression Texas-Red, RUNX2 expression FITC (scale bar represents 50 µm). e Graph displays the correlation between BOLCs and the number of TGFβ-positive cells ( p < 0.0001; R 2 0.865). Representative image of TGFβ cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 100 µm). f Graph shows the correlation between BOLCs and the number of vimentin-positive cells ( p < 0.0001; R 2 0.810). Representative image of vimentin cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 80 µm). g Graph displays the correlation between BOLCs and the percentage of Ki67-positive cells ( p < 0.0001, R 2 0.859). Representative image of Ki67 cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 100 µm). h Graph shows the correlation between BOLCs and the number of CD44-positive cells ( p < 0.0001, R 2 0.627). Representative image of CD44 cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 100 µm). i Graph displays the correlation between BOLCs and the number of ER-positive cells ( p < 0.0001, R 2 0.827). Representative image of ER cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 100 µm). j Graph shows the number of BOLCs in score 0, score 1, score 2 and score 3 groups (Her2 scoring system) ( p = 0.581). Representative image of HER2 cancer-positive cells in a breast-infiltrating carcinoma (scale bar represents 100 µm)

Article Snippet: Sections were then incubated for 1 h at room temperature with the following primary antibodies diluted 1:100: RUNX2 (clone 1D8, Novus Biologicals, USA), RANKL (clone 12A668, Abcam, Cambridge, UK) vimentin (clone 2D1, Novus Biologicals), TGFβ (clone 1D11.16.8, Ki67, Novus Biologicals), CD44 (clone 8E2F3, Novus Biologicals), ER (clone SP1, Novus Biologicals), and HER2 (clone 4B5, Ventana, Tucson, USA).

Techniques: Expressing

Journal: European Journal of Dentistry

Article Title: A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone

doi: 10.4103/ejd.ejd_342_17

Figure Lengend Snippet: The microscopic result of expression Runx2. Arrows show Runx2 expression on immunoreactive osteogenic cell

Article Snippet: Immunohistochemistry staining using monoclonal mouse TGF-β1 antibody (Novus Biologicals, USA) and monoclonal mouse Runx2 antibody (Novus Biologicals, USA) was conducted.

Techniques: Expressing

Journal: European Journal of Dentistry

Article Title: A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone

doi: 10.4103/ejd.ejd_342_17

Figure Lengend Snippet: Graph of mean value and standard deviation of each group on Runx2 expression. Different superscripts show a statistically significant difference ( P < 0.05)

Article Snippet: Immunohistochemistry staining using monoclonal mouse TGF-β1 antibody (Novus Biologicals, USA) and monoclonal mouse Runx2 antibody (Novus Biologicals, USA) was conducted.

Techniques: Standard Deviation, Expressing

Journal: Optics and Lasers in Engineering

Article Title: Hydrothermal treatment of laser micro-textured Ti6Al4V systems for osseointegration of orthopaedic implants

doi: 10.1016/j.optlaseng.2024.108511

Figure Lengend Snippet: Fig. 1. 3D (left) and 2D (middle) optical profilometry images of the GW60, GW90 and GW120 micro-pillar textured samples and fluorescence images of MSC culture (right). Fluorescence images of MSC correspond to the merge of three channels: Phalloidin staining (green fluorescence) highlighting the cytoskeleton of the cells, DAPI staining (blue fluorescence) demonstrating the nuclei of cells, and RUNX2 expression detected with brown fluorescence staining. All fluorescence images include a 100 μm scale bar.

Article Snippet: Then, cells were blocked with 3 % BSA in PBS for 1 h at room temperature and incubated at 4 ◦C overnight with rat anti-human antibody specific for RUNX2 (232902; R&D Systems, USA).

Techniques: Fluorescence, Staining, Expressing

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a ) Immunofluorescence images of Runx2 in BMSCs co-cultured with hydrogels during osteogenic differentiation. ( b ) Number of Runx2-positive cells in BMSCs co-cultured with hydrogels. *** p < 0.005; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques: Immunofluorescence, Cell Culture

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a – d ) qPCR analysis of OCN , COL-1 , Runx2 and Osterix in BMSCs on Day 7 and 14. * p < 0.05; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques: